ING2 Monoklonaler Antikörper

ING2 Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C3A7

Kat-Nr. : 66512-1-Ig

Synonyme

ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2

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Galerie der Validierungsdaten

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  WB analysis of HSC-T6 using 66512-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66512-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 66512-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 66512-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human ING2 (66512-1-Ig, Clone:2C3A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6-Zellen, NIH/3T3-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66512-1-Ig bindet in WB, FC, ELISA ING2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ING2 fusion protein Ag19291
• Vollständiger Name: inhibitor of growth family, member 2
• Berechnetes Molekulargewicht: 280 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC030128
• Gene symbol: ING2
• Gene ID (NCBI): 3622
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program.