IMPDH1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IMPDH1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 22092-1-AP

Synonyme

IMP dehydrogenase 1, IMPD, IMPD 1, IMPD1, IMPDH 1, IMPDH I, IMPDH1, LCA11, RP10, sWSS2608

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 22092-1-AP

  WB result of IMPDH1 antibody (22092-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH1 transfected HeLa cells.

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  WB analysis using 22092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22092-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 22092-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22092-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22092-1-AP

  IP Result of anti-IMPDH1 (IP:22092-1-AP, 4ug; Detection:22092-1-AP 1:2000) with HeLa cells lysate 3200ug.

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  IHC staining of human ovary tumor using 22092-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.

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  IHC staining of human ovary tumor using 22092-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 22092-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 22092-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 22092-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22092-1-AP (IMPDH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, K-562-Zellen, Mausmilzgewebe, Raji-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

22092-1-AP bindet in WB, IP, IHC, IF, ELISA IMPDH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IMPDH1 fusion protein Ag17297
• Vollständiger Name: IMP (inosine monophosphate) dehydrogenase 1
• Berechnetes Molekulargewicht: 563 aa, 60 kDa
• Beobachtetes Molekulargewicht: 53-60 kDa
• GenBank-Zugangsnummer: BC033622
• Gene symbol: IMPDH1
• Gene ID (NCBI): 3614
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa. This antibody may has cross reaction with IMPDH2 due to their high homology.

Publikationen

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB,IHC,IF,IP	Mol Ther; IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.; Authors - Hailong Ruan ; KD Validated
human	WB	J Oncol; The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses; Authors - Chang-Jun Liu ; KD Validated
human	WB	Cells; Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.; Authors - Madhu Kollareddy
human	WB	Int J Biochem Cell Biol; Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms.; Authors - Gunter Jennifer H JH
human	WB	Clin Chem Lab Med; Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies.; Authors - S John Calise