ILK Monoklonaler Antikörper

ILK Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E10E4

Kat-Nr. : 67724-1-Ig

Synonyme

DKFZp686F1765, ILK, ILK 1, ILK 2, ILK1, ILK2, integrin linked kinase, Integrin linked protein kinase, P59, p59ILK

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Galerie der Validierungsdaten

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  WB analysis using 67724-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of human peripheral blood platelets using 67724-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67724-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig colon using 67724-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 67724-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 67724-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, Hausschwein-Kolongewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, K-562-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67724-1-Ig bindet in WB, ELISA ILK und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ILK fusion protein Ag21289
• Vollständiger Name: integrin-linked kinase
• Berechnetes Molekulargewicht: 451 aa, 59 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC001554
• Gene symbol: ILK
• Gene ID (NCBI): 3611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).