CD122/IL2RB Monoklonaler Antikörper

CD122/IL2RB Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3A12C7

Kat-Nr. : 60174-1-Ig

Synonyme

CD122/IL2RB

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Galerie der Validierungsdaten

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  WB analysis using 60174-1-Ig

  Untreated and PNGase F-treated lysates of NK-92 cells were subjected to SDS PAGE followed by western blot with 60174-1-Ig (CD122/IL2RB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 60174-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60174-1-Ig (IL2RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NK-92 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60174-1-Ig bindet in WB, ELISA CD122/IL2RB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD122/IL2RB fusion protein Ag3929
• Vollständiger Name: interleukin 2 receptor, beta
• Berechnetes Molekulargewicht: 551 aa, 61 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 61 kDa
• GenBank-Zugangsnummer: BC025691
• Gene symbol: IL2RB
• Gene ID (NCBI): 3560
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.