IL-21R Monoklonaler Antikörper

IL-21R Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1C2

Kat-Nr. : 66319-1-Ig

Synonyme

CD360, IL 21 receptor, IL 21R, IL21R, IL-21R, interleukin 21 receptor, NILR, Novel interleukin receptor

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Galerie der Validierungsdaten

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  WB analysis using 66319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66319-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66319-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL21R Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, K-562-Zellen, MOLT-4-Zellen, TF-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66319-1-Ig bindet in WB, IHC,ELISA IL-21R und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-21R fusion protein Ag21295
• Vollständiger Name: interleukin 21 receptor
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC004348
• Gene symbol: IL21R
• Gene ID (NCBI): 50615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.