IL-1 Beta Monoklonaler Antikörper

IL-1 Beta Monoklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

CloneNo.

1C6F12

Kat-Nr. : 60136-1-Ig

Synonyme

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Galerie der Validierungsdaten

• 

  WB analysis of Recombinant protein using 60136-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60136-1-Ig (IL1 beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of RAW 264.7 using 60136-1-Ig

  IP Result of anti-IL1 beta (IP:60136-1-Ig, 5ug; Detection:60136-1-Ig 1:600) with RAW 264.7 cells lysate 4000ug.

• 

  IHC staining of human skin cancer using 60136-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60136-1-Ig (IL1 beta antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 60136-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60136-1-Ig (IL1 beta antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche IP: RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• ELISA: See 1 publications below

Produktinformation

60136-1-Ig bindet in WB, IP, IHC, ELISA IL-1 Beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-1 Beta fusion protein Ag10295
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 269 aa, 31 kDa
• GenBank-Zugangsnummer: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15 to 18 kDa protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.

Publikationen

Species	Application	Title
human	WB	Nat Immunol; RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.; Authors - Xiaqiong Wang
human,mouse	WB	Nat Cell Biol; Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity.; Authors - Yi Huang
human,mouse	WB	Nat Commun; CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.; Authors - Tiantian Tang
human	WB	EMBO Mol Med; Tranilast directly targets NLRP3 to treat inflammasome-driven diseases.; Authors - Yi Huang
human	WB	Oncotarget; Loss of AIM2 expression promotes hepatocarcinoma progression through activation of mTOR-S6K1 pathway.; Authors - Xiaomin Ma
rat	WB	J Ethnopharmacol; Yi-shen-hua-shi granules modulate immune and inflammatory damage via the ALG3/PPARγ/NF-κB pathway in the treatment of immunoglobulin a nephropathy; Authors - Rongjia Xu