IL-1 Alpha Polyklonaler Antikörper

IL-1 Alpha Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Kat-Nr. : 16765-1-AP

Synonyme

Hematopoietin 1, IL 1 alpha, IL 1A, IL1, IL-1, IL1 ALPHA, IL-1 alpha, IL1A, IL1F1, IL-1α, Interleukin 1 alpha, interleukin 1, alpha

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Galerie der Validierungsdaten

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  WB analysis using 16765-1-AP

  Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL-1 Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16765-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16765-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16765-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 16765-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16765-1-AP (IL-1 Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 16765-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16765-1-AP (IL-1 Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 16765-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 16765-1-AP (IL 1 alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, LPS and Brefeldin A treated THP-1 cells, humanes Plazenta-Gewebe, Mauslebergewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IHC: See 11 publications below
• IF: See 7 publications below
• ELISA: See 1 publications below

Produktinformation

16765-1-AP bindet in WB, IHC, IF, ELISA IL-1 Alpha und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-1 Alpha fusion protein Ag10339
• Vollständiger Name: interleukin 1, alpha
• Berechnetes Molekulargewicht: 271 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC013142
• Gene symbol: IL1A
• Gene ID (NCBI): 3552
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.

Publikationen

Species	Application	Title
mouse	IHC	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	IF	Nat Commun; Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment; Authors - Meilin Xue
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang
		Cancer Lett; Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer; Authors - Prabhakar Pitta Venkata
rat	WB	Bioact Mater; Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.; Authors - Liwen Luo
human	IF	Cell Death Discov; Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer.; Authors - Lulin Zhou