IL-19 Monoklonaler Antikörper

IL-19 Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

8H10H8

Kat-Nr. : 60278-1-Ig

Synonyme

IL 10C, IL 19, IL19, IL-19, interleukin 19, MDA1, NG.1, ZMDA1

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 60278-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60278-1-Ig (IL-19 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 60278-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60278-1-Ig (IL-19 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 60278-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 60278-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 60278-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of U-937 using 60278-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Hautkrebsgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, U-937-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60278-1-Ig bindet in IHC, IF, ELISA IL-19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-19 fusion protein Ag18887
• Vollständiger Name: interleukin 19
• Berechnetes Molekulargewicht: 177 aa, 20 kDa
• GenBank-Zugangsnummer: BC153127
• Gene symbol: IL19
• Gene ID (NCBI): 29949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-19, a member of the IL-10 cytokine family, is expressed in epithelial cells, endothelial cells, and macrophages. It can bind the interleukin-20 receptor complex IL-20R1/IL-20R2 and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). IL-19 induces IL-6 and TNF-alpha production in monocytes. It also induces cell apoptosis and reactive oxygen species production in monocytes, which suggests a role of this cytokine in inflammatory responses. It is up-regulated in monocytes following stimulation with lipopolysaccharide (LPS), and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-19 alters the balance of Th1 and Th2 cells in favor of Th2 cells. IL-19 contributes to a range of diseases and disorders, such as breast cancer, squamous cell carcinoma of the skin, asthma, endotoxic shock, uremia, psoriasis, rheumatoid arthritis, and periodontal and vascular disease.