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IL-18 Monoklonaler Antikörper

IL-18 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

5C6F8

Kat-Nr. : 60070-1-Ig

Synonyme

Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, pre-IL18



Geprüfte Anwendungen

Erfolgreiche Detektion in WBU2OS-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, humanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFhumanes Tonsillitisgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60070-1-Ig bindet in WB, IHC, IF, ELISA IL-18 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen IL-18 fusion protein Ag1063
Vollständiger Name IL-18
Berechnetes Molekulargewicht 193 aa, 22 kDa
Beobachtetes Molekulargewicht 24 kDa
GenBank-ZugangsnummerBC007461
Gene symbol IL18
Gene ID (NCBI) 3606
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.

Protokolle

Produktspezifische Protokolle
WB protocol for IL-18 antibody 60070-1-IgProtokoll herunterladen
IHC protocol for IL-18 antibody 60070-1-IgProtokoll herunterladen
IF protocol for IL-18 antibody 60070-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nature

Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome

Authors - Xuyan Shi
WB

J Neuroinflammation

GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome.

Authors - Zhangjin Qin
ratWB

J Ethnopharmacol

Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and Reperfusion--From the perspective of glial-vascular unit

Authors - Chang Zhou
humanIHC, WB

Int J Biol Sci

Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer.

Authors - Honglin Yan
WB

Front Microbiol

Methamphetamine Disturbs Gut Homeostasis and Reshapes Serum Metabolome, Inducing Neurotoxicity and Abnormal Behaviors in Mice.

Authors - Kai-Kai Zhang
WB

Liver Transpl

Endogenous Interleukin-18 Suppresses Liver Regeneration after Hepatectomy in Mice.

Authors - Tao Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bethany (Verified Customer) (01-05-2022)

Didn't work.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse kidney tissue