IL-13Rα1 Polyklonaler Antikörper

IL-13Rα1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 27916-1-AP

Synonyme

Cancer/testis antigen 19, CD213A1, CT19, IL 13 receptor subunit alpha 1, IL 13R alpha 1, IL 13R subunit alpha 1, IL 13Ra, IL 13RA1, IL13R, IL13RA, IL13RA1, IL-13RA1, IL-13Rα1, NR4

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Galerie der Validierungsdaten

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  WB analysis using 27916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27916-1-AP (IL-13RA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, Jurkat-Zellen, Maus-Skelettmuskelgewebe, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27916-1-AP bindet in WB, ELISA IL-13Rα1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-13Rα1 fusion protein Ag25542
• Vollständiger Name: interleukin 13 receptor, alpha 1
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC009960
• Gene symbol: IL13RA1
• Gene ID (NCBI): 3597
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a 65- to 70-kd glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ciara (Verified Customer) (01-30-2024)

Positive staining in N2a cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Neuro-2a mouse neuroblastoma cell line