IL-12/IL-23 p40 Monoklonaler Antikörper

IL-12/IL-23 p40 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

C17.8

Kat-Nr. : CL594-65097

Synonyme

Il 12b, Il 12p40, Il12b, Il12p40, interleukin 12b, p40

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Galerie der Validierungsdaten

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  FC experiment of mouse peritoneal macrophages using CL594-65097

  1X10^6 IFN-γ primed Balb/c mouse peritoneal macrophages were unstimulated or stimulated with LPS in the presence of a protein transport inhibitor and then intracellularly stained with 0.5 ug CoraLite®594 Anti-Mouse IL-12/IL-23 p40 (CL594-65097, Clone:C17.8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-65097 bindet in FC (Intra) IL-12/IL-23 p40 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHO-exprimiertes, rekombinantes Maus-IL-12 p70
• Vollständiger Name: interleukin 12b
• GenBank-Zugangsnummer: BC103608
• Gene symbol: Il12b
• Gene ID (NCBI): 16160
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).