IL-12/IL-23 p40 Monoklonaler Antikörper

IL-12/IL-23 p40 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

C17.8

Kat-Nr. : 65097-1-Ig

Synonyme

Il12b, Il-12b

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Galerie der Validierungsdaten

• 

  FC experiment of RAW 264.7 using 65097-1-Ig

  1X10^6 untreated or IFNγ, LPS, BFA and Monensin treated RAW 264.7 cells were intracellularly stained with 0.5 ug Anti-Mouse IL-12/IL-23 p40 (65097-1-Ig, Clone: C17.8) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: IFNγ, LPS, BFA and Monensin treated RAW 264.7 cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65097-1-Ig bindet in FC IL-12/IL-23 p40 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHO-exprimiertes, rekombinantes Maus-IL-12 p70
• Vollständiger Name: interleukin 12b
• GenBank-Zugangsnummer: BC103608
• Gene symbol: Il12b
• Gene ID (NCBI): 16160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

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