IKZF1 Monoklonaler Antikörper

IKZF1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1D6B5

Kat-Nr. : 66966-1-Ig

Synonyme

DNA binding protein Ikaros, hIk 1, Hs.54452, IK1, IKAROS, IKZF1, LYF1, PRO0758, ZNFN1A1

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Galerie der Validierungsdaten

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  WB analysis using 66966-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit spleen using 66966-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig thymus using 66966-1-Ig

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66966-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 66966-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, Hausschwein-Thymusgewebe, Jurkat-Zellen, Kaninchenmilzgewebe, MOLT-4-Zellen, Ramos-Zellen, Sp2/0-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

66966-1-Ig bindet in WB, ELISA IKZF1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IKZF1 fusion protein Ag28637
• Vollständiger Name: IKAROS family zinc finger 1 (Ikaros)
• Berechnetes Molekulargewicht: 477 aa, 53 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC018349
• Gene symbol: IKZF1
• Gene ID (NCBI): 10320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Transcriptional Regulation of PIK3CD and PIKFYVE in T-Cell Acute Lymphoblastic Leukemia by IKAROS and Protein Kinase CK2.; Authors - Elanora Dovat ; KD Validated