IGFBP4 Polyklonaler Antikörper

IGFBP4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 18500-1-AP

Synonyme

BP 4, HT29 IGFBP, IBP 4, IBP4, IGF binding protein 4, IGFBP 4, IGFBP4

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Galerie der Validierungsdaten

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  WB analysis of C6 using 18500-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 18500-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of C6 using 18500-1-AP

  Immunofluorescent analysis of C6 cells using 18500-1-AP (IGFBP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

18500-1-AP bindet in WB, IHC, IF, ELISA IGFBP4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Huhn
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGFBP4 fusion protein Ag13347
• Vollständiger Name: insulin-like growth factor binding protein 4
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-40 kDa
• GenBank-Zugangsnummer: BC016041
• Gene symbol: IGFBP4
• Gene ID (NCBI): 3487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401). IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.

Publikationen

Species	Application	Title
human	IF	Sci Adv; The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain.; Authors - Cynthia Hajal
human	WB	Theranostics; Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.; Authors - Mengmeng Qin
human	WB	BMC Biol; SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells; Authors - Yang Yang
human	IHC	Front Oncol; High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.; Authors - Wenwen Guo
chicken	WB	Cell Signal; PMID: 28734787; Authors - Jie Yang
human	WB	Oral Dis; FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization; Authors - Xiangrui Ma