IGFBP3 Monoklonaler Antikörper

IGFBP3 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A12A11

Kat-Nr. : 68402-1-Ig

Synonyme

BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 68402-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68402-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 68402-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68402-1-Ig (IGFBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68402-1-Ig bindet in WB, IHC, ELISA IGFBP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IGFBP3 fusion protein Ag20351
• Vollständiger Name: insulin-like growth factor binding protein 3
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC000013
• Gene symbol: IGFBP3
• Gene ID (NCBI): 3486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)