IGF1 Polyklonaler Antikörper

IGF1 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 28530-1-AP

Synonyme

IBP1, IGF I, IGF1, IGF1A, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C

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Galerie der Validierungsdaten

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  IHC staining of human liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Mauslebergewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

28530-1-AP bindet in WB, IHC, IF, Cell treatment, ELISA IGF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGF1 fusion protein Ag29197
• Vollständiger Name: insulin-like growth factor 1 (somatomedin C)
• Berechnetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_001111285.2
• Gene symbol: IGF1
• Gene ID (NCBI): 3479
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

Publikationen

Species	Application	Title
human	WB	Cancer Lett; Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner.; Authors - Jiao Li
human,mouse	WB	Metabolism; Arginine reverses growth hormone resistance through the inhibition of toll-like receptor 4-mediated inflammatory pathway.; Authors - Jingren Xu
	WB	Mol Nutr Food Res; Milk Polar Lipids Supplementation to Obese Rats During Pregnancy and Lactation Benefited Skeletal Outcomes of Male Offspring.; Authors - Lihua Han
mouse	WB	Int J Biol Sci; Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation; Authors - Yin Ni
mouse	IF	Biomed Pharmacother; Low-dose X-ray irradiation combined with FAK inhibitors improves the immune microenvironment and confers sensitivity to radiotherapy in pancreatic cancer.; Authors - Huanliang Chen
rat	WB	Biochem Cell Biol; Mechanism of myocardial fibrosis regulation by IGF-1R in atrial fibrillation through the PI3K/Akt/FoxO3a pathway; Authors - Pei Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hongxue (Verified Customer) (01-17-2023)

IHC staining for mouse liver, works well.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: mouse liver

IGF1 Antibody Immunohistochemistry validation (1:250 dilution) in mouse liver (Cat no:28530-1-AP)