IGBP1 Polyklonaler Antikörper

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• KD/KO Validated

IGBP1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14952-1-AP

Synonyme

ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 14952-1-AP

  WB result of IGBP1 antibody (14952-1-AP, 1:1000) with si-control and si-IGBP1 transfected Jurkat cells.

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  WB analysis of Raji using 14952-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14952-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14952-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14952-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 14952-1-AP

  IP result of anti-IGBP1 (IP:14952-1-AP, 4ug; Detection:14952-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Jurkat
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14952-1-AP bindet in WB, IP,ELISA IGBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IGBP1 fusion protein Ag6782
• Vollständiger Name: immunoglobulin (CD79A) binding protein 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC004137
• Gene symbol: IGBP1
• Gene ID (NCBI): 3476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.