IFT88 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

IFT88 Monoklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

4A4G5

Rezensionen (1)

Kat-Nr. : 60227-1-Ig

Synonyme

IFT88, TPR repeat protein 10, TTC10

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Galerie der Validierungsdaten

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  WB analysis of human testis using 60227-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 60227-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 60227-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 60227-1-Ig

  IP Result of anti-IFT88 (IP:60227-1-Ig, 5ug; Detection:60227-1-Ig 1:800) with PC-3 cells lysate 1040ug.

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  IHC staining of human kidney using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human testis, Gewebe
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

60227-1-Ig bindet in WB, IP, IHC, IF, ELISA IFT88 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFT88 fusion protein Ag4980
• Vollständiger Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 88-95 kDa
• GenBank-Zugangsnummer: BC030776
• Gene symbol: IFT88
• Gene ID (NCBI): 8100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Publikationen

Species	Application	Title
mouse	WB	J Cell Physiol; Crosstalk between the COX2-PGE2-EP4 signaling pathway and primary cilia in osteoblasts after mechanical stimulation.; Authors - Yan-Hui Li ; KD Validated
mouse	IF	Cell Cycle; Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.; Authors - Saul S Siller
human	WB	Front Cell Dev Biol; Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells.; Authors - Jiawei Li
mouse	WB	J Cell Physiol; Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.; Authors - Caleb S Hayes
human	WB	Molecules; 2-IPMA Ameliorates PM2.5-Induced Inflammation by Promoting Primary Ciliogenesis in RPE Cells; Authors - Ji Yeon Choi ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

k (Verified Customer) (10-26-2023)

I used it with TBST+5% BSA over night for WB analysis.

• Applications: Western Blot
• Primary Antibody Dilution: 1;500
• Cell Tissue Type: HePG2, Hela

IFT88 Antibody Western Blot validation (1;500 dilution) in HePG2, Hela (Cat no:60227-1-Ig)