IFN Gamma Monoklonaler Antikörper

IFN Gamma Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E10G7

Kat-Nr. : CL488-69007

Synonyme

IFG, IFI, IFN gamma, IFN γ, IFNG, IFN-gamma, IFNγ, IFN-γ, Immune interferon, Interferon gamma, interferon, gamma, NeutraKine® IFN Gamma

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using CL488-69007

  1X10^6 PMA+ionomycin+protein transport inhibitors treated for 6h human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human PBMCs using CL488-69007

  1X10^6 human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Jurkat using CL488-69007

  1X10^6 PMA and ionomycin treated Jurkat cells were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7) (red), or 0.25 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of NK92 using CL488-69007

  1X10^6 NK92 were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7) (red), or 0.25 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: 6 Stunden mit PMA+Ionomycin+Proteintransportinhibitoren stimulierte humane PBMCs, NK92, PMA and ionomycin treated Jurkat cells

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-69007 bindet in FC (Intra) IFN Gamma und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFN Gamma fusion protein HZ-1301
• Vollständiger Name: IFN gamma
• Gene symbol: IFNG
• Gene ID (NCBI): 3458
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C