IDO2 Polyklonaler Antikörper

IDO2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 25053-1-AP

Synonyme

IDO 2, IDO2, INDOL1, indoleamine 2,3 dioxygenase 2

---

Galerie der Validierungsdaten

• 

  WB analysis of A549 using 25053-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25053-1-AP (IDO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 25053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25053-1-AP (IDO2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 25053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25053-1-AP (IDO2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung using 25053-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25053-1-AP (IDO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 25053-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25053-1-AP (IDO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 25053-1-AP

  Immunofluorescent analysis of A549 cells using 25053-1-AP (IDO2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

25053-1-AP bindet in WB, IHC, IF, ELISA IDO2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IDO2 fusion protein Ag21866
• Vollständiger Name: indoleamine 2,3-dioxygenase 2
• Berechnetes Molekulargewicht: 407 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC113496
• Gene symbol: IDO2
• Gene ID (NCBI): 169355
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDO2, also named as INDOL1, catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. IDO2 gene has two isoforms with MW 45 kDa and 20 kDa.

Publikationen

Species	Application	Title
human	WB	Sci Signal; Kynurenine signaling through the aryl hydrocarbon receptor maintains the undifferentiated state of human embryonic stem cells.; Authors - Takako Yamamoto
human	WB	Toxicol Appl Pharmacol; Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.; Authors - Azubuike Peter Ebokaiwe
mouse	IF	FEBS J; Indoleamine 2,3-dioxygenase 2 deficiency associates with autism-like behavior via dopaminergic neuronal dysfunction; Authors - Masaki Ishikawa ; KO Validated
human	IHC	Cancer Med; Tryptophan metabolism enzymes are potential targets in ovarian clear cell carcinoma; Authors - Sumei Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hala (Verified Customer) (10-05-2020)

clean and no background when revealing

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: liver