IDE Polyklonaler Antikörper

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• KD/KO Validated

IDE Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 21728-1-AP

Synonyme

Abeta degrading protease, IDE, INSULYSIN

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Galerie der Validierungsdaten

• 

  WB analysis using 21728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21728-1-AP

  WB result of IDE antibody (21728-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells.

• 

  WB analysis of HepG2 using 21728-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 21728-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 21728-1-AP

  IP Result of anti-IDE (IP:21728-1-AP, 4ug; Detection:21728-1-AP 1:500) with HepG2 cells lysate 1600ug.

• 

  IHC staining of human kidney using 21728-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21728-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 21728-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 21728-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, DU 145-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below

Produktinformation

21728-1-AP bindet in WB, IP, IHC, ELISA IDE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IDE fusion protein Ag16392
• Vollständiger Name: Abeta degrading protease
• Berechnetes Molekulargewicht: 1019 aa, 118 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC096336
• Gene symbol: IDE
• Gene ID (NCBI): 3416
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDE (INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531). This antibody is specific to IDE.

Publikationen

Species	Application	Title
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
human	WB	Carbohydr Polym; A pectin from fruits of Lycium barbarum L. decreases β-amyloid peptide production through modulating APP processing.; Authors - Lishuang Zhou
human	WB	Cell Physiol Biochem; Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.; Authors - Qin Zheng
human	WB	Int J Biol Macromol; A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.; Authors - Hui Zeng
human	WB	J Biol Chem; Cell surface expression of the major amyloid-β peptide (Aβ)-degrading enzyme, neprilysin, depends on phosphorylation by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) and dephosphorylation by protein phosphatase 1a.; Authors - Naomasa Kakiya
	WB	Neurochem Int; Activation of α7 nicotinic acetylcholine receptor alleviates Aβ1-42-induced neurotoxicity via downregulation of p38 and JNK MAPK signaling pathways.; Authors - Ke-Wei Chang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (12-15-2020)

Non-specific band at around 60kDa was stronger than band for IDE, although IDE band could still be made out.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

IDE Antibody Western Blot validation (1:500 dilution) in HEK293T (Cat no:21728-1-AP)