ICAM2 Monoklonaler Antikörper

ICAM2 Monoklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, FC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1H3C6

Kat-Nr. : 67628-1-Ig

Synonyme

CD102, ICAM 2, ICAM2

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Galerie der Validierungsdaten

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  WB analysis of human peripheral blood platelets using 67628-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67628-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HUVEC using 67628-1-Ig

  1X10^6 HUVEC cells were stained with 0.2 ug Anti-Human ICAM2 (67628-1-Ig, Clone:1H3C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug mouse IgG2a isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood platelets, Jurkat-Zellen
• Erfolgreiche Detektion in FC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67628-1-Ig bindet in WB, FC,ELISA ICAM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ICAM2 fusion protein Ag29441
• Vollständiger Name: intercellular adhesion molecule 2
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC003097
• Gene symbol: ICAM2
• Gene ID (NCBI): 3384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.