IARS2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IARS2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17170-1-AP

Synonyme

IARS2, IleRS, Isoleucine tRNA ligase

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Galerie der Validierungsdaten

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  WB analysis using 17170-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17170-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17170-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17170-1-AP

  IP Result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with HeLa cells lysate 4000ug.

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  IP experiment of Jurkat using 17170-1-AP

  IP Result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with Jurkat cells lysate 4000ug.

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  IHC staining of human liver cancer using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17170-1-AP

  Immunofluorescent analysis of HepG2 cells, using IARS2 antibody 17170-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslungengewebe
• Erfolgreiche IP: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

17170-1-AP bindet in WB, IP, IHC, IF, ELISA IARS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IARS2 fusion protein Ag10696
• Vollständiger Name: isoleucyl-tRNA synthetase 2, mitochondrial
• Berechnetes Molekulargewicht: 1012 aa, 114 kDa
• Beobachtetes Molekulargewicht: 114 kDa
• GenBank-Zugangsnummer: BC010218
• Gene symbol: IARS2
• Gene ID (NCBI): 55699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

Publikationen

Species	Application	Title
human	IF, WB	Mol Cell; Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.; Authors - Sung-Hoon Kim
human	WB	Discov Oncol; High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer; Authors - Haiqing Wang
human	WB	Rev Assoc Med Bras (1992); IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells.; Authors - Yue-Ming Yu
human	WB	Front Oncol; The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.; Authors - Xin Di ; KD Validated