IARS Polyklonaler Antikörper

IARS Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 26942-1-AP

Synonyme

IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, isoleucyl tRNA synthetase, PRO0785

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Galerie der Validierungsdaten

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  WB analysis using 26942-1-AP

  K-562 cells and U2OS cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26942-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26942-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 26942-1-AP

  IP result of anti-IARS (IP:26942-1-AP, 4ug; Detection:26942-1-AP 1:300) with U2OS cells lysate 1800 ug.

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  IHC staining of human testis using 26942-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 26942-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 26942-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 26942-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, U2OS-Zellen
• Erfolgreiche IP: U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

26942-1-AP bindet in WB, IP, IHC, IF, ELISA IARS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IARS fusion protein Ag25686
• Vollständiger Name: isoleucyl-tRNA synthetase
• Berechnetes Molekulargewicht: 144 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC065552
• Gene symbol: IARS
• Gene ID (NCBI): 3376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Food Chem Toxicol; The crucial role of thioredoxin interacting protein in the liver insulin resistance induced by di (2-ethylhexyl) phthalates.; Authors - Wang Zhang
mouse	WB	Sci Rep; Intermittent fasting activates macrophage migration inhibitory factor and alleviates high-fat diet-induced nonalcoholic fatty liver disease; Authors - Dezhao Li
human	WB,IF	Discov Oncol; High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer; Authors - Haiqing Wang
human	IF	Nat Commun; Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle; Authors - Shuyao Hu
human	WB	Proc Natl Acad Sci U S A; Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex; Authors - Krishnendu Khan ; KD Validated