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  • Featured Product
  • KD/KO Validated

HuR/ELAVL1 Polyklonaler Antikörper

HuR/ELAVL1 Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-11910

Synonyme

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated HuR/ELAVL1 Polyclonal anti (CL594-11910)

    FC experiment of MCF-7 using CL594-11910

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human HuR/ELAVL1 (CL594-11910) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-11910 bindet in FC (Intra) HuR/ELAVL1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HuR/ELAVL1 fusion protein Ag2249
Vollständiger Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Berechnetes Molekulargewicht 326 aa, 36 kDa
Beobachtetes Molekulargewicht 30-37 kDa
GenBank-ZugangsnummerBC003376
Gene symbol ELAVL1
Gene ID (NCBI) 1994
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protokolle

Produktspezifische Protokolle
FC protocol for CL594 HuR/ELAVL1 antibody CL594-11910Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen