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  • Featured Product
  • KD/KO Validated

HuR/ELAVL1 Polyklonaler Antikörper

HuR/ELAVL1 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11910

Synonyme

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated HuR/ELAVL1 Polyclona (CL488-11910)

    IF Staining of MCF-7 using CL488-11910

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 HuR/ELAVL1 antibody (CL488-11910) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HuR/ELAVL1 Polyclona (CL488-11910)

    IF Staining of HeLa using CL488-11910

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HuR antibody (CL488-11910) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated HuR/ELAVL1 Polyclona (CL488-11910)

    FC experiment of MCF-7 using CL488-11910

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human HuR/ELAVL1 (CL488-11910) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen, MCF-7-Zellen
Erfolgreiche Detektion in FCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11910 bindet in IF, FC (Intra) HuR/ELAVL1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HuR/ELAVL1 fusion protein Ag2249
Vollständiger Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Berechnetes Molekulargewicht 326 aa, 36 kDa
Beobachtetes Molekulargewicht 30-37 kDa
GenBank-ZugangsnummerBC003376
Gene symbol ELAVL1
Gene ID (NCBI) 1994
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 HuR/ELAVL1 antibody CL488-11910Protokoll herunterladen
FC protocol for CL Plus 488 HuR/ELAVL1 antibody CL488-11910Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen