Histone H2B Monoklonaler Antikörper

Histone H2B Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Huhn, human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1E12G3

Kat-Nr. : 68393-1-Ig

Synonyme

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

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Galerie der Validierungsdaten

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  WB analysis using 68393-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68393-1-Ig (Histone H2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 68393-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68393-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68393-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H2B antibody (68393-1-Ig, Clone: 1E12G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 68393-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H2B antibody (68393-1-Ig, Clone: 1E12G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 68393-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H2B (68393-1-Ig, Clone:1E12G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Zebrafischgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68393-1-Ig bindet in WB, IHC, IF, FC, ELISA Histone H2B und zeigt Reaktivität mit Huhn, human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: Huhn, human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 2, H2be
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14-17 kDa
• GenBank-Zugangsnummer: BC005827
• Gene symbol: HIST2H2BE
• Gene ID (NCBI): 8349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).

Publikationen

Species	Application	Title
human	WB	Clin Epigenetics; Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis; Authors - Xiangjun Yin