HVCN1 Polyklonaler Antikörper

HVCN1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14162-1-AP

Synonyme

HV1, HVCN1, VSOP

---

Galerie der Validierungsdaten

• 

  WB analysis of Raji using 14162-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14162-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14162-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 14162-1-AP

  IP result of anti-HVCN1 (IP:14162-1-AP, 4ug; Detection:14162-1-AP 1:300) with PC-3 cells lysate 4000 ug.

• 

  IHC staining of human tonsillitis using 14162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsillitis using 14162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of PC-3 using 14162-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 14162-1-AP (HVCN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

14162-1-AP bindet in WB, IP, IHC, IF,ELISA HVCN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HVCN1 fusion protein Ag5350
• Vollständiger Name: hydrogen voltage-gated channel 1
• Berechnetes Molekulargewicht: 273 aa, 32 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa, ~60 kDa
• GenBank-Zugangsnummer: BC032672
• Gene symbol: HVCN1
• Gene ID (NCBI): 84329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW ~60 kDa.

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Diagnostic and Predictive Value of Immune-Related Genes in Crohn's Disease.; Authors - Bing Yu