HTRA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HTRA1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 55011-1-AP

Synonyme

ARMD7, High-temperature requirement A serine peptidase 1, HtrA, HtrA serine peptidase 1, HTRA1, L56, ORF480, PRSS11, Serine protease 11, Serine protease HTRA1

---

Galerie der Validierungsdaten

• 

  WB analysis of HAP1 cells using 55011-1-AP

  HAP1 (WT and HTRA1 KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis of L02 using 55011-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55011-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HAP1 cells using 55011-1-AP

  HAP1 lysates prepared and IP of APP performed using 2.0 μg of 55011-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  IP experiment of L02 using 55011-1-AP

  IP Result of anti-HTRA1 (IP:55011-1-AP, 4ug; Detection:55011-1-AP 1:500) with L02 cells lysate 1400ug.

• 

  IHC staining of human placenta using 55011-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 55011-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 55011-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 55011-1-AP

  Immunofluorescent analysis of HepG2 cells using 55011-1-AP (HTRA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HepG2-Zellen, Maushirngewebe
• Erfolgreiche IP: L02-Zellen, HAP1 cells
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

55011-1-AP bindet in WB, IP, IHC, IF, ELISA HTRA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: HtrA serine peptidase 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 35 kDa, 50 kDa, 42 kDa
• GenBank-Zugangsnummer: NM_002775
• Gene symbol: HTRA1
• Gene ID (NCBI): 5654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

Publikationen

Species	Application	Title
mouse		Biomaterials; Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm; Authors - Craig Jun Kit Wong
rat	WB,IF	Front Immunol; Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling.; Authors - Hong Xiang
human	IHC,IF	FASEB J; The "Matrisome" reveals the characterization of skin keloid microenvironment.; Authors - Shikun Zhang
Zebrafish	IHC,WB	Am J Pathol; High Temperature Requirement A 1 (HTRA1) Causes Photoreceptor Cell Death in Zebrafish Disease Models.; Authors - Yoshihito Oura
human	IHC	Am J Pathol; Proteolytic Degradation and Inflammation Play Critical Roles in Polypoidal Choroidal Vasculopathy.; Authors - Sandeep Kumar
mouse	WB	Bioengineered; Long non-coding RNA lincRNA-erythroid prosurvival (EPS) alleviates cerebral ischemia/reperfusion injury by maintaining high-temperature requirement protein A1 (Htra1) stability through recruiting heterogeneous nuclear ribonucleoprotein L (HNRNPL).; Authors - Haifeng Guo