HTR2B Polyklonaler Antikörper

HTR2B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 26408-1-AP

Synonyme

5 HT 2B, 5 HT(2B), 5 HT2B, HTR2B, Serotonin receptor 2B

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 26408-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26408-1-AP (HTR2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 26408-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26408-1-AP (HTR2B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 26408-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26408-1-AP (HTR2B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 26408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26408-1-AP (HTR2B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26408-1-AP (HTR2B Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

26408-1-AP bindet in WB, IHC, ELISA HTR2B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HTR2B fusion protein Ag24007
• Vollständiger Name: 5-hydroxytryptamine (serotonin) receptor 2B
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC063123
• Gene symbol: HTR2B
• Gene ID (NCBI): 3357
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serotonin (5-HT) gene has been implicated in the development of personality traits or temperament predisposition. HTR2B(serotonin receptor 2B) has been shown that 3,4-methylene-dioxymethamphetamine, commonly referred to as the drug ecstasy, selectively binds and activates 5-HT2B receptors to induce serotonin release in mouse raphe nuclei, which leads to dopamine release in the nucleus accumbens and ventral tegmentum (PMID:23774082). Moreover, One of the most reliable predictive markers of UM (Uveal melanoma) at risk of evolving toward the formation of liver lesions is an abnormally elevated level of expression of the transcript encoding the HTR2B (PMID:31002821).

Publikationen

Species	Application	Title
human	WB,IHC	Hypertension; Subtypes of Histopathologically Classical Aldosterone-Producing Adenomas Yield Various Transcriptomic Signaling and Outcomes.; Authors - Vin-Cent Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anna (Verified Customer) (10-04-2022)

Large background, no specific reaction. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

• Applications: Immunofluorescence
• Primary Antibody Dilution: 500, 1000, 2000
• Cell Tissue Type: brain frozen sections - mouse