HSPB8 Polyklonaler Antikörper

HSPB8 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15287-1-AP

Synonyme

Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, E2 induced gene 1 protein, E2IG1, H11, heat shock 22kDa protein 8, Heat shock protein beta 8, HMN2, HMN2A, HSP22, HSPB8, Protein kinase H11

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Galerie der Validierungsdaten

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  WB analysis using 15287-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15287-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 15287-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15287-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 15287-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, HEK-293-Zellen, Mausherzgewebe, Maushirngewebe, Rattenherzgewebe, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15287-1-AP bindet in WB, IHC, IF, CoIP, ELISA HSPB8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPB8 fusion protein Ag7456
• Vollständiger Name: heat shock 22kDa protein 8
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-22 kDa
• GenBank-Zugangsnummer: BC002673
• Gene symbol: HSPB8
• Gene ID (NCBI): 26353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, IF	Nat Commun; Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.; Authors - Thomas G Martin
mouse	IHC	J Neurosci; The α crystallin domain of small heat shock protein b8 (Hspb8) acts as survival and differentiation factor in adult hippocampal neurogenesis.; Authors - Ramírez-Rodríguez Gerardo G
mouse	WB	J Cell Biochem; Pharmacological inhibition of BAG3-HSP70 with the proposed cancer therapeutic JG-98 is toxic for cardiomyocytes.; Authors - Thomas G Martin
mouse,human	CoIP	Genes (Basel); BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.; Authors - Hongtao Diao
human	WB	Cell Rep; Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR.; Authors - Dawid Krokowski
human	WB,IHC	Biochem Cell Biol; HSPB8 facilitates prostate cancer progression via activating the JAK/STAT3 signaling pathway; Authors - Kan Zhang