HSP20 Polyklonaler Antikörper

HSP20 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16813-1-AP

Synonyme

Heat shock protein beta 6, Hsp20, HSPB6

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Galerie der Validierungsdaten

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  WB analysis using 16813-1-AP

  rat heart tissues were subjected to SDS PAGE followed by western blot with 16813-1-AP (HSP20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16813-1-AP (HSP20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 16813-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16813-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16813-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16813-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 16813-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using HSP20 antibody (16813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16813-1-AP bindet in WB, IHC, IF, ELISA HSP20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSP20 fusion protein Ag10352
• Vollständiger Name: heat shock protein, alpha-crystallin-related, B6
• Berechnetes Molekulargewicht: 160 aa, 17 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC068046
• Gene symbol: HSPB6
• Gene ID (NCBI): 126393
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.