HSPA1L Monoklonaler Antikörper

HSPA1L Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

CloneNo.

3E7H2

Kat-Nr. : 66780-1-Ig

Synonyme

Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66780-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66780-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66780-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66780-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66780-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66780-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 66780-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66780-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66780-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66780-1-Ig (HSPA1L antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, L02-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66780-1-Ig bindet in WB, IHC, IF,ELISA HSPA1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSPA1L fusion protein Ag5182
• Vollständiger Name: heat shock 70kDa protein 1-like
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC034483
• Gene symbol: HSPA1L
• Gene ID (NCBI): 3305
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognizes the endogenous HSPA1L protein in multiple cell lines. (21730052)