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HSP47 Monoklonaler Antikörper

HSP47 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A8F6

Kat-Nr. : CL488-67863

Synonyme

47 kDa heat shock protein, AsTP3, CBP1, CBP2, Collagen binding protein, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    IF Staining of HepG2 using CL488-67863

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HSP47 antibody (CL488-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    IF Staining of HeLa using CL488-67863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HSP47 antibody (CL488-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    IF Staining of HeLa using CL488-67863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HSP47 antibody (CL488-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    FC experiment of HepG2 using CL488-67863

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human HSP47 (CL488-67863, Clone:2A8F6) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen, HeLa-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67863 bindet in IF, FC (Intra) HSP47 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen HSP47 fusion protein Ag30999
Vollständiger Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Berechnetes Molekulargewicht 46 kDa
Beobachtetes Molekulargewicht46 kDa
GenBank-ZugangsnummerBC014623
Gene symbol SERPINH1
Gene ID (NCBI) 871
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 HSP47 antibody CL488-67863Protokoll herunterladen
FC protocol for CL Plus 488 HSP47 antibody CL488-67863Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen