HSP27 Monoklonaler Antikörper

HSP27 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E7A1

Kat-Nr. : CL488-66767

Synonyme

28 kDa heat shock protein, CMT2F, DKFZp586P1322, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HMN2B, HS.76067, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27

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Galerie der Validierungsdaten

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  IF Staining of MCF-7 using CL488-66767

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 HSP27 antibody (CL488-66767, Clone: 3E7A1 ) at dilution of 1:200.

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  FC experiment of MCF-7 using CL488-66767

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HSP27 (CL488-66767, Clone:3E7A1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66767 bindet in IF, FC (Intra) HSP27 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP27 fusion protein Ag27859
• Vollständiger Name: heat shock 27kDa protein 1
• Berechnetes Molekulargewicht: 19-23 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC012768
• Gene symbol: HSPB1
• Gene ID (NCBI): 3315
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C