HSF4 Polyklonaler Antikörper

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• KD/KO Validated

HSF4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18797-1-AP

Synonyme

CTM, Heat shock factor protein 4, hHSF4, HSF 4, HSF4, HSTF 4

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Galerie der Validierungsdaten

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  WB analysis using 18797-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18797-1-AP (HSF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18797-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18797-1-AP (HSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 18797-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18797-1-AP (HSF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 18797-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18797-1-AP (HSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below

Produktinformation

18797-1-AP bindet in WB, ELISA HSF4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: heat shock transcription factor 4
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53-58 kDa
• GenBank-Zugangsnummer: BC130383
• Gene symbol: HSF4
• Gene ID (NCBI): 3299
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSF4 belongs to the HSF family. HSF4 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE). The isoform HSF4A represses transcription while the isoform HSF4B activates transcription. HSF4 is a homotrimer which exhibits constitutive DNA binding and forms trimers even in the absence of stress. It is expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. Mutation of HSF4 will cause lamellar cataract which is known as Marner type cataract (CAM). Lamellar cataract is an autosomal dominant common type of infantile cataract. Finger malformation is observed in some kindreds. Inheritance of these forms of cataract is autosomal dominant.

Publikationen

Species	Application	Title
human	WB	Oncogene; FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression.; Authors - Feng Guo ; KD Validated
human	WB	Mol Cell Biochem; HSF4 promotes tumor progression of colorectal cancer by transactivating c-MET; Authors - Wenjing Zhang ; KD Validated
mouse	WB	Invest Ophthalmol Vis Sci; HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation; Authors - Jing Zhang