HSF1 Monoklonaler Antikörper

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• KD/KO Validated

HSF1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1F10A5

Kat-Nr. : 67189-1-Ig

Synonyme

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

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Galerie der Validierungsdaten

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  WB analysis using 67189-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67189-1-Ig (HSF1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HeLa using 67189-1-Ig

  WB result of HSF1 antibody (67189-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells.

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  IHC staining of human breast cancer using 67189-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67189-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67189-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67189-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67189-1-Ig bindet in WB, IHC, IF, ELISA HSF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSF1 fusion protein Ag29061
• Vollständiger Name: heat shock transcription factor 1
• Berechnetes Molekulargewicht: 529 aa, 57 kDa
• Beobachtetes Molekulargewicht: 68-80 kDa
• GenBank-Zugangsnummer: BC014638
• Gene symbol: HSF1
• Gene ID (NCBI): 3297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

Publikationen

Species	Application	Title
human	IHC,IF	Pancreas; Heat Shock Factor 1 in Relation to Tumor Angiogenesis and Disease Progression in Patients With Pancreatic Cancer.; Authors - Qiuman Lin