HSF1 Polyklonaler Antikörper

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• KD/KO Validated

HSF1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 51034-1-AP

Synonyme

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

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Galerie der Validierungsdaten

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  WB analysis using 51034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51034-1-AP

  WB result of HSF1 antibody (51034-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells.

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  WB analysis using 51034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 51034-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 51034-1-AP

  IP result of anti-HSF1(IP:51034-1-AP, 4ug; Detection:51034-1-AP 1:8000) with Jurkat cells lysate 1560 ug.

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  IHC staining of human lung cancer using 51034-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51034-1-AP (HSF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 51034-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51034-1-AP (HSF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 51034-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51034-1-AP (HSF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 51034-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51034-1-AP (HSF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 51034-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using HSF1 antibody (51034-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, C6-Zellen, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Maus-Eierstockgewebe, Maushodengewebe, NIH/3T3-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

51034-1-AP bindet in WB, IP, IHC, IF, ELISA HSF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSF1 fusion protein Ag0739
• Vollständiger Name: heat shock transcription factor 1
• Berechnetes Molekulargewicht: 529 aa, 57 kDa
• Beobachtetes Molekulargewicht: 68-80 kDa
• GenBank-Zugangsnummer: BC014638
• Gene symbol: HSF1
• Gene ID (NCBI): 3297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

Publikationen

Species	Application	Title
mouse	WB	Science; Z-DNA binding protein 1 promotes heatstroke-induced cell death.; Authors - Fangfang Yuan
mouse	WB	Nat Commun; Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy; Authors - Donghai Cui
rat		Int J Pharm; Effective myocardial infarction treatment by targeted accumulation of Sulforaphane using porous magnetic silica nanoparticles; Authors - Jian Zhang
human	WB	Am J Physiol Cell Physiol; Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1; Authors - Jie Zhao
mouse	WB	J Gerontol A Biol Sci Med Sci; Multifactorial Attenuation of the Murine Heat Shock Response With Age.; Authors - Donald A Jurivich
mouse	WB,IHC	Biogerontology; Disrupted HSF1 regulation in normal and exceptional brain aging; Authors - Rachana Trivedi