HSDL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HSDL2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15631-1-AP

Synonyme

C9orf99, HSDL2, SDR13C1

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Galerie der Validierungsdaten

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  WB analysis using 15631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15631-1-AP (HSDL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15631-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSDL2 antibody (15631-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

15631-1-AP bindet in WB, IHC, IF, FC, ELISA HSDL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSDL2 fusion protein Ag7987
• Vollständiger Name: hydroxysteroid dehydrogenase like 2
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC004331
• Gene symbol: HSDL2
• Gene ID (NCBI): 84263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

Publikationen

Species	Application	Title
human	WB	Proteomics; Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.; Authors - Songlin Zeng
human	IF	Cancer Manag Res; Ectopic expression of HSDL2 is related to cell proliferation and prognosis in breast cancer.; Authors - Bing Dong
human	IHC,WB	Onco Targets Ther; HSDL2 Acts as a Promoter in Pancreatic Cancer by Regulating Cell Proliferation and Lipid Metabolism.; Authors - Anna Han ; KD Validated
human	WB,FC	Chin Med; Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression.; Authors - Wen-Bei Liu ; KD Validated
human	IHC	Nan Fang Yi Ke Da Xue Xue Bao; [HSDL2 overexpression promotes rectal cancer progression by regulating cancer cell cycle and promoting cell proliferation]; Authors - Y Cheng ; KD Validated
human	WB,IHC	BMC Cancer; MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell; Authors - Ming Li