HSD17B4 Polyklonaler Antikörper

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• KD/KO Validated

HSD17B4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 15116-1-AP

Synonyme

17 beta HSD 4, D bifunctional protein, DBP, EDH17B4, HSD17B4, MFE 2, MPF2, Multifunctional protein 2, SDR8C1

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Galerie der Validierungsdaten

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  WB analysis using 15116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15116-1-AP

  WB result of HSD17B4 antibody (15116-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B4 transfected HepG2 cells.

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  WB analysis of mouse brain using 15116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15116-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15116-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15116-1-AP

  IP Result of anti-HSD17B4 (IP:15116-1-AP, 4ug; Detection:15116-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 15116-1-AP

  Immunofluorescent analysis of Hela cells, using HSD17B4 antibody 15116-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, Mausherzgewebe, Maushirngewebe, Rattenlebergewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, Mausherzgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below

Produktinformation

15116-1-AP bindet in WB, IP, IHC, IF, ELISA HSD17B4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD17B4 fusion protein Ag7165
• Vollständiger Name: hydroxysteroid (17-beta) dehydrogenase 4
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 45 kDa
• GenBank-Zugangsnummer: BC003098
• Gene symbol: HSD17B4
• Gene ID (NCBI): 3295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).

Publikationen

Species	Application	Title
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
mouse	WB	Neurobiol Dis; Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation.; Authors - Verheijden Simon S
	WB	Sci Rep; Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.; Authors - Mitsuki Nozaki
mouse	WB	Cells; Cell Type-Selective Loss of Peroxisomal β-Oxidation Impairs Bipolar Cell but Not Photoreceptor Survival in the Retina.; Authors - Daniëlle Swinkels
human	WB	Mol Cells; The Peroxisomal Localization of Hsd17b4 Is Regulated by Its Interaction with Phosphatidylserine.; Authors - Sang-Ah Lee
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali