HSD17B11 Polyklonaler Antikörper

HSD17B11 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 16303-1-AP

Synonyme

17 beta HSD 11, 17 beta HSD XI, 17 BETA HSD11, 17 BETA HSDXI, 17betaHSD11, 17betaHSDXI, 17bHSD11, DHRS8, HSD17B11, PAN1B, RETSDR2, SDR16C2, UNQ207/PRO233

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Galerie der Validierungsdaten

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  WB analysis of L02 using 16303-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16303-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16303-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16303-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16303-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16303-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 16303-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16303-1-AP (HSD17B11 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16303-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16303-1-AP (HSD17B11 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Mausherzgewebe, Mauslebergewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16303-1-AP bindet in WB, IHC, ELISA HSD17B11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD17B11 fusion protein Ag9278
• Vollständiger Name: hydroxysteroid (17-beta) dehydrogenase 11
• Berechnetes Molekulargewicht: 300 aa, 33 kDa
• Beobachtetes Molekulargewicht: 28-33 kDa
• GenBank-Zugangsnummer: BC021673
• Gene symbol: HSD17B11
• Gene ID (NCBI): 51170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Biosci; m6A demethylase FTO promotes tumor progression via regulation of lipid metabolism in esophageal cancer.; Authors - Xiaoran Duan