HSD11B2 Polyklonaler Antikörper

HSD11B2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 14192-1-AP

Synonyme

11 beta HSD2, 11 DH2, AME, AME1, HSD11B2, HSD11K, HSD2, SDR9C3

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Galerie der Validierungsdaten

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  WB analysis using 14192-1-AP

  Human placenta tissue lysates were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14192-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14192-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14192-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14192-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14192-1-AP (HSD11B2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14192-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14192-1-AP (HSD11B2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Nierengewebe, Mausnierengewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Produktinformation

14192-1-AP bindet in WB, IHC, IF, ELISA HSD11B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD11B2 fusion protein Ag5146
• Vollständiger Name: hydroxysteroid (11-beta) dehydrogenase 2
• Berechnetes Molekulargewicht: 405 aa, 44 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC036780
• Gene symbol: HSD11B2
• Gene ID (NCBI): 3291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The HSD11B2 gene encodes the type II isoform of 11-beta-hydroxysteroid dehydrogenase, a microsomal enzyme complex responsible for the interconversion of biologically active cortisol and inactive cortisone. This protein catalyzes the cortisol-to-cortisone reaction. This protein expresses approximately 44 kDa in placenta, trophoblast, and distal colon, but in kidney tissue, there are two bands of approximately 44 and 48 kDa can be consistently observed and no signal is seen in decidua, adrenal, or liver(PMID:9048640). HSD11B2 protein was detected in CEM-C7 cells via immunoblot analysis as a dimer and trimer of approximately 80 and 120 kDa (PMID:23762608).

Publikationen

Species	Application	Title
rat	WB	Nutrients; Maternal High-Fructose Corn Syrup Intake Impairs Corticosterone Clearance by Reducing Renal 11β-Hsd2 Activity via miR-27a-Mediated Mechanism in Rat Offspring; Authors - Yuki Nouchi
rat	WB	Oncotarget; Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.; Authors - Shuai Lian
mouse	WB	Ecotoxicol Environ Saf; Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse.; Authors - Huiduo Guo
human	WB,IHC	Carcinogenesis; Modulation of the oral glucocorticoid system during black raspberry mediated oral cancer chemoprevention.; Authors - Divya Nedungadi ; KD Validated
pig	WB	Sci Rep; Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets.; Authors - Haodong Chen
rat	WB	Brain Res; Response of the maternal hypothalamus to cold stress during late pregnancy in rats.; Authors - Di Wang