HRPT2, CDC73 Monoklonaler Antikörper

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• KD/KO Validated

HRPT2, CDC73 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

4B4H2

Kat-Nr. : 66490-1-Ig

Synonyme

C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66490-1-Ig

  WB result of HRPT2, CDC73 antibody (66490-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HeLa cells.

• 

  WB analysis using 66490-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 66490-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 66490-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66490-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66490-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66490-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66490-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2; CDC73 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66490-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, LNCaP-Zellen, Maushirngewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

66490-1-Ig bindet in WB, IHC, IF, ELISA HRPT2, CDC73 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HRPT2, CDC73 fusion protein Ag2959
• Vollständiger Name: cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 531 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC014351
• Gene symbol: CDC73
• Gene ID (NCBI): 79577
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).

Publikationen

Species	Application	Title
mouse	WB	J Proteomics; To explore the regulatory role of Wnt/P53/Caspase3 signal in mouse ovarian development based on LFQ proteomics; Authors - Yabo Ma
mouse	WB	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao
	IHC	Horm Res Paediatr; A Familial Case of a Whole Germline CDC73 Deletion Discordant for Primary Hyperparathyroidism.; Authors - Naomi Hatabu