HPSE Polyklonaler Antikörper

HPSE Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16673-1-AP

Synonyme

Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1

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Galerie der Validierungsdaten

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  WB analysis of DU 145 using 16673-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 16673-1-AP (HPSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 16673-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 16673-1-AP (HPSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney slide using 16673-1-AP (HPSE Antibody) at dilution of 1:50.

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  IHC staining of mouse kidney using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney slide using 16673-1-AP (HPSE Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

16673-1-AP bindet in WB, IHC, IF,ELISA HPSE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HPSE fusion protein Ag10167
• Vollständiger Name: heparanase
• Berechnetes Molekulargewicht: 543 aa, 61 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 50 kDa
• GenBank-Zugangsnummer: BC051321
• Gene symbol: HPSE
• Gene ID (NCBI): 10855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.

Publikationen

Species	Application	Title
human,mouse	WB,IF	Cell Rep; Viral Activation of Heparanase Drives Pathogenesis of Herpes Simplex Virus-1.; Authors - Alex M Agelidis
mouse	WB	Diabetes Res Clin Pract; Heparanase-driven inflammation from the AGEs-stimulated macrophages changes the functions of glomerular endothelial cells.; Authors - Guang Xu