HOXD13 Polyklonaler Antikörper

HOXD13 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 18736-1-AP

Synonyme

BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD

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Galerie der Validierungsdaten

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  WB analysis of A549 using 18736-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18736-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 18736-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18736-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18736-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 18736-1-AP

  IP Result of anti-HOXD13 (IP:18736-1-AP, 4ug; Detection:18736-1-AP 1:500) with A549 cells lysate 1600ug.

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  IHC staining of human brain using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslungengewebe, Rattenhirngewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18736-1-AP bindet in WB, IP, IHC, ELISA HOXD13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: homeobox D13
• Berechnetes Molekulargewicht: 343 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC148863
• Gene symbol: HOXD13
• Gene ID (NCBI): 3239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOXD13, also named as HOX4I, belongs to the Abd-B homeobox family. HOXD13 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Mutation of HOXD13 will cause synpolydactyly (SPD), brachydactyly type D (BDD), syndactyly type 5brachydactyly-syndactyly syndrome (BDSD) or brachydactyly type E (BDE).Present polyclonal anti-HOX13D antibody(18736-1-AP) is produced by immunizing animals with part of N-terminus chain of HOX13D and detect a 36-kDa band in cell and tissues.

Publikationen

Species	Application	Title
		Mol Pharm; Sialic Acid-Functionalized PEG-PLGA Microspheres Loading Mitochondrial Targeting Modified Curcumin for Acute Lung Injury Therapy.; Authors - Fei-Yang Jin
mouse	WB	Cell Death Dis; A novel Hoxd13 mutation causes synpolydactyly and promotes osteoclast differentiation by regulating pSmad5/p65/c-Fos/Rank axis; Authors - Lishan Zhang