HOOK3 Polyklonaler Antikörper

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• KD/KO Validated

HOOK3 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 15457-1-AP

Synonyme

h hook3, hHK3, HK3, hook homolog 3 (Drosophila), HOOK3, Protein Hook homolog 3

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Galerie der Validierungsdaten

• 

  WB analysis of rat liver using 15457-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15457-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15457-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15457-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15457-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15457-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15457-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15457-1-AP

  IP Result of anti-HOOK3 (IP:15457-1-AP, 4ug; Detection:15457-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human kidney using 15457-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15457-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15457-1-AP

  Immunofluorescent analysis of HepG2 cells, using HOOK3 antibody 15457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, HEK-293-Zellen, humanes Lebergewebe, Mausherzgewebe, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15457-1-AP bindet in WB, IP, IHC, IF, ELISA HOOK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOOK3 fusion protein Ag7724
• Vollständiger Name: hook homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC056146
• Gene symbol: HOOK3
• Gene ID (NCBI): 84376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport.; Authors - Nida Siddiqui
rat	WB	Dev Cell; The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance.; Authors - Noopur V Khobrekar
human	WB,IP	J Cell Biol; Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.; Authors - Agnieszka A Kendrick
rat	WB	J Cell Biol; Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons.; Authors - Mara A Olenick
mouse	WB	Mol Cell Proteomics; Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation; Authors - Anne-Marie Pauwels
human	WB	iScience; Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.; Authors - Inbal Wortzel ; KO Validated