HNRPLL Polyklonaler Antikörper

HNRPLL Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 26769-1-AP

Synonyme

hnRNPLL, HNRPLL, SRRF, Stromal RNA regulating factor

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Galerie der Validierungsdaten

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  WB analysis using 26769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26769-1-AP (HNRPLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26769-1-AP (HNRPLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 26769-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26769-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26769-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26769-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26769-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26769-1-AP (HNRPLL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

26769-1-AP bindet in WB, IHC, IF, ELISA HNRPLL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRPLL fusion protein Ag25287
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein L-like
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC017480
• Gene symbol: HNRPLL
• Gene ID (NCBI): 92906
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRPLL, also named as HNRNPLL and SRRF, is an RNA binding protein that regulates alternative splicing of pre-mRNA. HnRNPLL is up-regulated in stimulated T cells, bound CD45 transcripts, and is a key inducible regulator of CD45 alternative splicing (PMID: 18669861). HNRPLL has 5 isoforms with the molecular weights of 31-60 kDa. Additionally, studies have shown that HNRPLL is a metastasis suppressor gene in colorectal cancer (PMID: 28360095).

Publikationen

Species	Application	Title
human	WB	Stem Cell Rev Rep; Lnc-PPP2R1B Mediates the Alternative Splicing of PPP2R1B by Interacting and Stabilizing HNRNPLL and Promotes Osteogenesis of MSCs; Authors - Shuping Peng
human	WB,IHC,IF	J Exp Clin Cancer Res; Energy stress-induced circZFR enhances oxidative phosphorylation in lung adenocarcinoma via regulating alternative splicing; Authors - Zhifei Ma ; KD Validated