HNRNPR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HNRNPR Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15018-1-AP

Synonyme

hnRNP R, HNRNPR, HNRPR

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15018-1-AP (HNRNPR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15018-1-AP (HNRNPR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 15018-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15018-1-AP (HNRNPR antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15018-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15018-1-AP (HNRNPR antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15018-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15018-1-AP (HNRNPR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

15018-1-AP bindet in WB, IHC, IF, ELISA HNRNPR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPR fusion protein Ag7028
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein R
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 65-82 kDa
• GenBank-Zugangsnummer: BC001449
• Gene symbol: HNRNPR
• Gene ID (NCBI): 10236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)

Publikationen

Species	Application	Title
human	WB	J Immunol; HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.; Authors - Adi Reches ; KD Validated
mouse	WB	Neurosci Lett; hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity.; Authors - Akinsola Raphael Akinyemi
human	IHC,WB	Front Oncol; The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.; Authors - Yixin Li
	WB	iScience; Increased LDL receptor by SREBP2 or SREBP2-induced lncRNA LDLR-AS promotes triglyceride accumulation in fish; Authors - Xiufei Cao ; KD Validated
human	IHC	J Transl Med; High expression of HNRNPR in ESCA combined with 18F-FDG PET/CT metabolic parameters are novel biomarkers for preoperative diagnosis of ESCA; Authors - Xiao-Yu Liu
human	WB	Eur J Pharm Sci; Novel facile thermosensitive hydrogel as sustained and controllable gene release vehicle for breast cancer treatment.; Authors - Dan Zhao