HMMR-Specific Polyklonaler Antikörper

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• KD/KO Validated

HMMR-Specific Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-15820

Synonyme

CD168, HMMR, HMMR-Specific, IHABP, RHAMM

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Galerie der Validierungsdaten

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  FC experiment of HepG2 using CL555-15820

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human HMMR-Specific (CL555-15820) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-15820 bindet in FC (Intra) HMMR-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: hyaluronan-mediated motility receptor (RHAMM)
• Berechnetes Molekulargewicht: 84 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_001142556
• Gene symbol: HMMR
• Gene ID (NCBI): 3161
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMMR, also named RHAMM, is a hyaluronan receptor expressed in various tissues. It is involved in regulation of focal contacts and subsequent motility, thus playing roles in wound healing and tumor metastasis. Remarkably different molecular masses of RHAMM have been published. Antibodies raised against RHAMM have been reported to recognize proteins of 56, 66 and 70 kDa in the supernatant of murine 3T3 fibroblasts(PMID: 2440472). Some of the isoforms detectable by anti-RHAMM antibodies, including the 52 kDa form and a 48-49 kDa form found in 10T1/2 cells, have been speculated to be underglycosylated precursor forms of the mature RHAMM protein(PMID: 1705559).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C