HMHA1 Polyklonaler Antikörper

HMHA1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14832-1-AP

Synonyme

HA 1, HLA HA1, HMHA1, KIAA0223, mHag HA 1

---

Galerie der Validierungsdaten

• 

  WB analysis using 14832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14832-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 14832-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14832-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsil using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsil using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillengewebe, humanes Milzgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

14832-1-AP bindet in WB, IHC, IF, ELISA HMHA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMHA1 fusion protein Ag6636
• Vollständiger Name: histocompatibility (minor) HA-1
• Berechnetes Molekulargewicht: 1136 aa, 125 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC065223
• Gene symbol: HMHA1
• Gene ID (NCBI): 23526
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Aging Dis; Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke; Authors - Hongyu Ma